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Table 2 Summary of the primers used in each project and sequencing success rates

From: Insights into biodiversity sampling strategies for freshwater microinvertebrate faunas through bioblitz campaigns and DNA barcoding

Project Primary primers Secondary primers Number of specimens Success rate (%) Number of specimens >200 bp with <2% Ns
CHUBL LCO1490_t1/HCO2198_t1 None 94 87% 78
SAOST LCO1490_t1/HCO2198_t1 LCO1490_t1/MLepR1; MLepF1/HCO2198_t1a 95 83% 79
COCSA LCO1490_t1/HCO2198_t1 CrustDF1/CrustDR1 190 65% 124
OZFWZb C_LepFolF/C_LepFolR ZplankF1_t1/ZplankR1_t1 90 70% 63
OZFWC – Plates 1 and 2b ZplankF1_t1/ZplankR1_t1 C_LepFolF/C_LepFolR 70 34% 24
OZFWC – Plates 3 and 4b LCO1490_t1/HCO2198_t1 C_LepFolF/C_LepFolR 49 37% 18
OZFWC – Plates 5-9b C_LepFolF/C_LepFolR ZplankF1_t1/ZplankR1_t1 164 68% 112
Combined    752 66.2% 498
  1. Primer sequences and references are provided in Additional file 1: Appendix A. The primer pairs used for both PCR amplification and cycle sequencing for each individual specimen are available through BOLD.
  2. a This primer combination involving mini-primers uses two separate PCR reactions to attempt to amplify the full-length barcode region in two fragments.
  3. b These samples were run on 96-well plates including mixed microcrustaceans, and thus the sample sizes do not reflect full plates.