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Figure 2 | BMC Ecology

Figure 2

From: Ecological comparison of cellular stress responses among populations – normalizing RT-qPCR values to investigate differential environmental adaptations

Figure 2

Hsp70A gene expression in air exposed individuals from all subpopulations, relative to control animals. Values normalized to reference genes Grp78 and HIF-1 for subpopulations in Puerto Montt (PM), Punta Arenas (PA) sublittoral and intertidal, Potter Cove (PC) sublittoral and intertidal. Asterisks indicate significant up- or down-regulation compared to the control animals (Bootstrap test by REST 2009, *p < 0.05, **p < 0.01, ***p < 0.001). Boxes represent 50% of values with median value (dotted line), whiskers are extreme values.

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