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Figure 4 | BMC Ecology

Figure 4

From: Ecological comparison of cellular stress responses among populations – normalizing RT-qPCR values to investigate differential environmental adaptations

Figure 4

Constitutive levels of gene expression for Hsp isoforms Hsp70A, Hsp70B and Hsc70 in Nacella subpopulations. Values of control animals, relative to Puerto Montt subpopulation and normalized to reference genes Grp78 and β-actin, for subpopulations at Puerto Montt (PM), Punta Arenas (PA) sublittoral and intertidal, Potter Cove (PC) sublittoral and intertidal. Letters indicate significant differentiation among the subpopulations. Asterisks indicate significant up-regulation compared to sublittoral animals at the same site (Bootstrap test by REST 2009, **p < 0.01). Boxes represent 50% of values with median value (dotted line), whiskers are extreme values.

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