Fig. 12From: A metabarcoding framework for facilitated survey of endolithic phototrophs with tufADatabase performance. Distribution of OTU’s best hit against the database for phototrophs and for the Ulvophyceae-only (orders Bryopsidales and ‘Ulvales-Ulothrichales’). ‘ALL’ represents matches for all OTUs regardless of abundance (in dark grey). ‘≥1 %’ represents matches for the most abundant OTUs (in light grey). Note the skewed distribution toward high identity matches for the most abundant OTUs (regardless of taxonomy) indicating high performance of the database for annotation of the targeted communities. Values were computed based on 375 bp of the tufA metabarcodeBack to article page