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Table 1 Newly designed PCR primers for tufA barcoding and metabarcoding

From: A metabarcoding framework for facilitated survey of endolithic phototrophs with tufA

Name

Sequence (5′–3′)

bp

GC ( %)

Tm ( °C)

tu470F

TTTTAATGGCTGTCGAAAATGTTG

24

33.3

52.8

tubryoF

GCAGATGGTCCAATGCCWCAAAC

23

52.2

59

tubryoR

CCWGGTTTAGCTAAAACCATNCC

23

45.7

54.9

env_tufAFa

TGGGTDGAHAADATTTWYNMNYTRATGR

28

33.3

46.5–62.8

env_tufARa

TNACATCHGTWGTWCKNACATARAAYTG

28

35.1

49.8–60.6

  1. a Metabarcoding primers