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Table 2 Limestone samples processed via cloning and metabarcoding

From: A metabarcoding framework for facilitated survey of endolithic phototrophs with tufA

Sample

Clon./Metab.a

Site

Depth (m)

Date

Substratum

FL01

−/+

Big pine key, Florida

4

09/2013

Reef rubble

FL02

−/+

Big pine key, Florida

4

09/2013

Reef rubble

JP01

+/−

Ryukyusc

<5

07/2012

Reef rubble

JP03

+/−

Ryukyus

<5

07/2012

Reef rubble

JP04

+/−

Ryukyus

<5

07/2012

Reef rubble

JP06

+/−

Ryukyus

<5

07/2012

Reef matrix

JP07

+/+

Ryukyus

<5

07/2012

Reef matrix/rubble

JP25

+/−

Ryukyus

<5

07/2012

Reef rubble

GM14

−/+

Ewing Bank, NWGMb

57

08/2008

Rhodolith

E09

+/−

–

–

03/2013

Aquarium window

S15

+/−

NWGM

65

08/2008

Lithophyllum sp.

  1. a Cloning and/or metabarcoding: performed (+), not performed (−)
  2. b Northwestern Gulf of Mexico
  3. c Mixed locations and sampling dates within the archipelago