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Table 3 Remaining reads throughout the UPARSE pipeline as counts and percentage of raw reads

From: A metabarcoding framework for facilitated survey of endolithic phototrophs with tufA

Raw

4,917,888

100 %

Paired-ends merging

4,720,138

96

Demultiplexing

1,928,898

39

Read quality filter

1,465,438

30

Read size filtera

1,331,438

27

Denoisingb

837,069

17

Decontaminationc

824,355

17

  1. a Reads used for OTU mapping
  2. b Singletons, doubletons and tripletons
  3. c Non-tufA reads and chimeric reads